Optical-NIR analysis of globular clusters in the IKN dwarf spheroidal: a complex star formation history

Age, metallicity and spatial distribution of globular clusters (GCs) provide a powerful tool to reconstruct major star-formation episodes in galaxies. IKN is a faint dwarf spheroidal (dSph) in the M81 group of galaxies. It contains five old GCs, which makes it the galaxy with the highest known specific frequency (SN=126). We estimate the photometric age, metallicity and spatial distribution of the poorly studied IKN GCs. We search SDSS for GC candidates beyond the HST field of view, which covers half of IKN. To break the age-metallicity degeneracy in the V-I colour we use WHT/LIRIS Ks-band photometry and derive photometric ages and metallicities by comparison with SSP models in the V,I,Ks colour space. IKN GCs' VIKs colours are consistent with old ages ($\geq\!8$ Gyr) and a metallicity distribution with a higher mean than typical for such a dSph ([Fe/H$]\!\simeq\!-1.4_{-0.2}^{+0.6}$ dex). Their photometric masses range ($0.5 <{\cal M_{\rm GC}}<4\times10^5M_\odot$) implies a high mass ratio between GCs and field stars, of $10.6\%$. Mixture model analysis of the RGB field stars' metallicity suggests that 72\% of the stars may have formed together with the GCs. Using the most massive GC-SFR relation we calculate a SFR of $\sim\!10M_\odot/$yr during its formation epoch. We note that the more massive GCs are closer to the galaxy photometric centre. IKN GCs also appear spatially aligned along a line close to the IKN major-axis and nearly orthogonal to the plane of spatial distribution of galaxies in the M81 group. We identify one new IKN GC candidate based on colour and PSF analysis of the SDSS data. The evidence towards i) broad and high metallicity distribution of the field IKN RGB stars and its GCs, ii) high fraction and iii), spatial alignment of IKN GCs, supports a scenario for tidally triggered complex IKN's SFH in the context of interactions with galaxies in the M81 group.Comment: 12 pages, 9 figures, accepted to A&

High resolution imaging of the early-type galaxy NGC 1380: an insight into the nature of extended extragalactic star clusters

NGC 1380 is a lenticular galaxy located near the centre of the Fornax Cluster northeast of NGC 1399. The globular cluster system of this galaxy was previously studied only from the ground. Recent studies of similar early-type galaxies, specially lenticular ones, reveal the existence of star clusters that apparently break up the traditional open/globular cluster dichotomy. With higher quality photometry from HST/WFPC2 we study the star clusters in NGC 1380, measuring their magnitudes, colours, sizes and projected distances from the centre of the galaxy. We used deep archival HST/WFPC2 in the B and V bands. We built colour magnitude diagrams from which we selected a sample of cluster candidates. We also analysed their colour distribution and measured their sizes. Based on their location in the luminosity-size diagram we estimated probabilities of them being typical globular clusters as those found in the Galaxy. A total of about 570 cluster candidates were found down to V=26.5. We measured sizes for approximately 200 of them. The observed colour distribution has three apparent peaks. Likewise for the size distribution. We identified the smaller population as being mainly typical globular clusters, while the more extended objects have small probabilities of being such objects. Different correlations between absolute magnitudes, sizes, colours and location were inferred for these cluster sub-populations. Most extended clusters (Reff > 4 pc) share similar properties to the diffuse star clusters reported to inhabit luminous early-type galaxies in the Virgo galaxy cluster such as being of low surface brightness and fainter than MV ~ -8. We also report on a small group of (Reff ~ 10 pc), -8< MV < -6, red clusters located near the centre of NGC 1380, which may be interpreted as faint fuzzies.Comment: accepted for publication in A&

Wavelength self-calibration and sky subtraction for Fabry–Pérot interferometers: applications to OSIRIS

We describe techniques concerning wavelength calibration and sky subtraction to maximize the scientific utility of data from tunable filter instruments. While we specifically address data from the Optical System for Imaging and low Resolution Integrated Spectroscopy instrument (OSIRIS) on the 10.4-m Gran Telescopio Canarias telescope, our discussion is generalizable to data from other tunable filter instruments. A key aspect of our methodology is a coordinate transformation to polar coordinates, which simplifies matters when the tunable filter data are circularly symmetric around the optical centre. First, we present a method for rectifying inaccuracies in the wavelength calibration using OH sky emission rings. Using this technique, we improve the absolute wavelength calibration from an accuracy of ∼5 to 1 Å, equivalent to ∼7 per cent of our instrumental resolution, for 95 per cent of our data. Then, we discuss a new way to estimate the background sky emission by median filtering in polar coordinates. This method suppresses contributions to the sky background from the outer envelopes of distant galaxies, maximizing the fluxes of sources measured in the corresponding sky-subtracted images. We demonstrate for data tuned to a central wavelength of 7615 Å that galaxy fluxes in the new sky-subtracted image are ∼37 per cent higher, versus a sky-subtracted image from existing methods for OSIRIS tunable filter data

The Overlooked Potential of Generalized Linear Models in Astronomy-III: Bayesian Negative Binomial Regression and Globular Cluster Populations

In this paper, the third in a series illustrating the power of generalized linear models (GLMs) for the astronomical community, we elucidate the potential of the class of GLMs which handles count data. The size of a galaxy's globular cluster population $N_{\rm GC}$ is a prolonged puzzle in the astronomical literature. It falls in the category of count data analysis, yet it is usually modelled as if it were a continuous response variable. We have developed a Bayesian negative binomial regression model to study the connection between $N_{\rm GC}$ and the following galaxy properties: central black hole mass, dynamical bulge mass, bulge velocity dispersion, and absolute visual magnitude. The methodology introduced herein naturally accounts for heteroscedasticity, intrinsic scatter, errors in measurements in both axes (either discrete or continuous), and allows modelling the population of globular clusters on their natural scale as a non-negative integer variable. Prediction intervals of 99% around the trend for expected $N_{\rm GC}$comfortably envelope the data, notably including the Milky Way, which has hitherto been considered a problematic outlier. Finally, we demonstrate how random intercept models can incorporate information of each particular galaxy morphological type. Bayesian variable selection methodology allows for automatically identifying galaxy types with different productions of GCs, suggesting that on average S0 galaxies have a GC population 35% smaller than other types with similar brightness.Comment: 14 pages, 12 figures. Accepted for publication in MNRA

An optical/NIR survey of globular clusters in early-type galaxies III. On the colour bimodality of GC systems

The interpretation that bimodal colour distributions of globular clusters (GCs) reflect bimodal metallicity distributions has been challenged. Non-linearities in the colour to metallicity conversions caused by the horizontal branch (HB) stars may be responsible for transforming a unimodal metallicity distribution into a bimodal (optical) colour distribution. We study optical/near-infrared (NIR) colour distributions of the GC systems in 14 E/S0 galaxies. We test whether the bimodal feature, generally present in optical colour distributions, remains in the optical/NIR ones. The latter colour combination is a better metallicity proxy than the former. We use KMM and GMM tests to quantify the probability that different colour distributions are better described by a bimodal, as opposed to a unimodal distribution. We find that double-peaked colour distributions are more commonly seen in optical than in optical/NIR colours. For some of the galaxies where the optical (g-z) distribution is clearly bimodal, the (g-K) and (z-K) distributions are better described by a unimodal distribution. The two most cluster-rich galaxies in our sample, NGC4486 and NGC4649, show some interesting differences. The (g-K) distribution of NGC4649 is better described by a bimodal distribution, while this is true for the (g-K) distribution of NGC4486 GCs only if restricted to a brighter sub-sample with small K-band errors (< 0.05 mag). Formally, the K-band photometric errors cannot be responsible for blurring bimodal metallicity distributions to unimodal (g-K) colour distributions. However, simulations including the extra scatter in the colour-colour diagrams (not fully accounted for in the photometric errors) show that such scatter may contribute to the disappearance of bimodality in (g-K) for the full NGC4486 sample. For the less cluster-rich galaxies results are inconclusive due to poorer statistics. [Abridged]Comment: A&A accepted, 15 pages, 10 figures, 4 table

The impact of environmental effects on AGN: a decline in the incidence of ionized outflows

AGN have been generally considered to be less frequent in denser environments due to the lower number of galaxy-galaxy interactions and/or the removal of their gas-rich reservoirs by the dense intergalactic medium. However, recent observational and theoretical works suggest that the effect of ram-pressure stripping might reduce the angular momentum of their gas, causing it to infall towards the super massive black hole (SMBH) at their centre, activating the AGN phase. In this work we explore the connection between environment and nuclear activity by evaluating the variation in the incidence of ionized outflows in AGN across different environments. We select a sample of $\sim3300$ optical AGN from the Sloan Digital Sky Survey Data Release 13 that we match with the group catalogue from Lim et al. 2017. We further probe their environment through the projected distance to the central galaxy of the group/cluster and the projected surface density to the 5th neighbour ($\delta_5$). We find that at lower masses ($<10^{10.3}$M$_{\odot}$), the fraction of ionized outflows is significantly lower in satellite ($\sim7$%) than in isolated ($\sim22$%) AGN. The fraction of outflows in all satellite AGN decreases towards closer distances to the central, whereas only the lower-mass ones display a significant decline with $\delta_5$. Although this study does not include AGN in the densest regions of galaxy clusters, our findings suggest that AGN in dense environments accrete less gas than those in the field potentially due to the removal of the gas reservoirs via stripping or starvation, consistent with a negative connection between environment and AGN activity. We propose that the observed change in the incidence of outflows towards denser regions of groups and clusters could contribute to the higher gas metallicities of cluster galaxies compared to field ones, especially at lower masses.Comment: 9 pages, 6 figures, Accepted for publication in Astronomy and Astrophysic

Human granulocyte colony stimulating factor (hG-CSF): cloning, overexpression, purification and characterization

<p>Abstract</p> <p>Background</p> <p>Biopharmaceutical drugs are mainly recombinant proteins produced by biotechnological tools. The patents of many biopharmaceuticals have expired, and biosimilars are thus currently being developed. Human granulocyte colony stimulating factor (hG-CSF) is a hematopoietic cytokine that acts on cells of the neutrophil lineage causing proliferation and differentiation of committed precursor cells and activation of mature neutrophils. Recombinant hG-CSF has been produced in genetically engineered <it>Escherichia coli </it>(Filgrastim) and successfully used to treat cancer patients suffering from chemotherapy-induced neutropenia. Filgrastim is a 175 amino acid protein, containing an extra N-terminal methionine, which is needed for expression in <it>E. coli</it>. Here we describe a simple and low-cost process that is amenable to scaling-up for the production and purification of homogeneous and active recombinant hG-CSF expressed in <it>E. coli </it>cells.</p> <p>Results</p> <p>Here we describe cloning of the human granulocyte colony-stimulating factor coding DNA sequence, protein expression in <it>E. coli </it>BL21(DE3) host cells in the absence of isopropyl-β-D-thiogalactopyranoside (IPTG) induction, efficient isolation and solubilization of inclusion bodies by a multi-step washing procedure, and a purification protocol using a single cationic exchange column. Characterization of homogeneous rhG-CSF by size exclusion and reverse phase chromatography showed similar yields to the standard. The immunoassay and N-terminal sequencing confirmed the identity of rhG-CSF. The biological activity assay, <it>in vivo</it>, showed an equivalent biological effect (109.4%) to the standard reference rhG-CSF. The homogeneous rhG-CSF protein yield was 3.2 mg of bioactive protein per liter of cell culture.</p> <p>Conclusion</p> <p>The recombinant protein expression in the absence of IPTG induction is advantageous since cost is reduced, and the protein purification protocol using a single chromatographic step should reduce cost even further for large scale production. The physicochemical, immunological and biological analyses showed that this protocol can be useful to develop therapeutic bioproducts. In summary, the combination of different experimental strategies presented here allowed an efficient and cost-effective protocol for rhG-CSF production. These data may be of interest to biopharmaceutical companies interested in developing biosimilars and healthcare community.</p

The metal-poor dwarf irregular galaxy candidate next to Mrk 1172

In this work we characterise the properties of the object SDSS J020536.84-081424.7, an extended nebular region with projected extension of $14 \times 14$ kpc$^{2}$ in the line of sight of the ETG Mrk 1172, using unprecedented spectroscopic data from MUSE. We perform a spatially resolved stellar population synthesis and estimate the stellar mass for both Mrk 1172 ($1 \times 10^{11} M_{\odot}$) and our object of study ($3 \times 10^{9} M_{\odot}$). While the stellar content of Mrk 1172 is dominated by an old ($\sim 10$ Gyr) stellar population, the extended nebular emission has its light dominated by young to intermediate age populations (from $\sim 100$ Myr to $\sim 1$ Gyr) and presents strong emission lines such as: H${\beta}$, [O III] ${\lambda}{\lambda}$4959,5007, H${\alpha}$, [N II] ${\lambda}{\lambda}$6549,6585 and [S II] ${\lambda}{\lambda}$6717,6732. Using these emission lines we find that it is metal-poor (with $Z \sim$ 1/3 $Z_{\odot}$, comparable to the LMC) and is actively forming stars ($0.70$ M$_{\odot}$ yr$^{-1}$), especially in a few bright clumpy knots that are readily visible in H${\alpha}$. The object has an ionised gas mass $\geq 3.8 \times 10^{5}$ M$_{\odot}$. Moreover, the motion of the gas is well described by a gas in circular orbit in the plane of a disk and is being affected by interaction with Mrk 1172. We conclude that SDSS J020536.84-081424.7 is most likely a dwarf irregular galaxy (dIGal)

Optical/NIR stellar absorption and emission-line indices from luminous infrared galaxies

We analyze a set of optical-to-near-infrared long-slit nuclear spectra of 16 infrared-luminous spiral galaxies. All of the studied sources present H$_2$ emission, which reflects the star-forming nature of our sample, and they clearly display H I emission lines in the optical. Their continua contain many strong stellar absorption lines, with the most common features due to Ca I, Ca II, Fe I, Na I, Mg I, in addition to prominent absorption bands of TiO, VO, ZrO, CN and CO. We report a homogeneous set of equivalent width (EW) measurements for 45 indices, from optical to NIR species for the 16 star-forming galaxies as well as for 19 early type galaxies where we collected the data from the literature. This selected set of emission and absorption-feature measurements can be used to test predictions of the forthcoming generations of stellar population models. We find correlations among the different absorption features and propose here correlations between optical and NIR indices, as well as among different NIR indices, and compare them with model predictions. While for the optical absorption features the models consistently agree with the observations,the NIR indices are much harder to interpret. For early-type spirals the measurements agree roughly with the models, while for star-forming objects they fail to predict the strengths of these indices.Comment: accepted for publication in MNRA